Epithelial expression of TNFAIP3 induces a Th1-type colitis in IL-10KO mice. ELISAs were performed on the colonic mucosa from IL-10KO vs. TNFAIP3tg × IL-10KO mice, and these revealed elevated levels of IFN-γ (A) and reduced levels of IL-4 (B) typical of a Th1-type of inflammation. There were also significantly reduced levels of IL-22 (C), thymic stromal lymphopoietin (TSLP) (F), and IL-25 (G) and a trend toward reduced IL-6 (H), in the mucosa of TNFAIP3tg × IL-10KO mice, compared with IL-10KO mice. No differences were observed in the mucosal levels of TNF (D), IL-12/23 (E), or IL-17 (I). *P < 0.05.