Table 2.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Source/reference |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Research Genetics, Inc. |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Research Genetics, Inc. |
| yAM135-A | BY4741 Ypk1(L424A)::URA3-ypk2Δ::KanMX4 | This study |
| JTY6142 | BY4741 ypk1Δ::KanMX4 | Research Genetics, Inc. |
| yAM120-A | BY4741 ypk2Δ::KanMX4 | This study |
| yAM159-A | BY4741 3xFLAG-Lag1::LEU2 | This study |
| yAM163-A | BY4741 3xFLAG-Lag1(S23A S24A)::LEU2 | This study |
| yAM165-A | BY4742 3xHA-Lac1::HIS3 | This study |
| yAM166-A | BY4742 3xHA-Lac1 (S23A S24A)::HIS3 | This study |
| JTY5574 | BY4741 cna1Δ::KanMX4 cna2Δ::KanMX4 | M.S. Cyert, Stanford Univ. |
| YDB379 | BY4741 Ypk1-3xFLAG::natNT2 | J.S. Weissman, Univ. of California, San Francisco |
| yAM205-A | BY4742 Lac1::LEU2 Lag1::LEU2 | This study |
| yAM207-B | BY4742 Lac1(S23A S24A)::LEU2 Lag1(S23A S24A)::LEU2 | This study |
| yAM210 | BY4742 Lac1(S23E S24E)::LEU2 Lag1(S23E S24E)::LEU2 | This study |
| yGT12 | BY4742 LYS2+ Lac1::LEU2 Lag1::LEU2 lcb3Δ::natNT2 | This study |
| yGT13 | BY4742 LYS2+ Lac1(S23A S24A)::LEU2 Lag1(S23A S24A)::LEU2 lcb3Δ::natNT2 | This study |
| yGT14 | BY4742 LYS2+ Lac1(S23E S24E)::LEU2 Lag1(S23E S24E)::LEU2 lcb3Δ::natNT2 | This study |
| yAM168 | BY4741 3xHA-Lac1::HIS3 3xFLAG-Lag1::LEU2 | This study |
| yAM184 | BY4741 3xHA-Lac1(S23A S24A)::HIS3 3xFLAG-Lag1(S23A S24A)::LEU2 | This study |
| yAM192-A | BY4741 MET15+ 3xHA-Lac1(S23E S24E)::HIS3 3xFLAG-Lag1(S23E S24E)::LEU2 | This study |
| yKL4 | BY4741 TOR2+::Hygr | Kristin Leskoske, this lab |
| yKL5 | BY4741 Tor2(L2178A)::Hygr | Kristin Leskoske, this lab |