Table 3.
Plasmids used in this study
| Plasmid | Description | Source/reference |
|---|---|---|
| pGEX6P-1 | GST tag, bacterial expression vector | GE Healthcare, Inc. |
| pGEX4T-1 | GST tag, bacterial expression vector | GE Healthcare, Inc. |
| YCpLG | CEN, LEU2, PGAL1 vector | (Bardwell et al., 1998) |
| BG1805 | 2 µm, URA3, PGAL1, C-terminal tandem affinity (TAP) tag vector | Open Biosystems, Inc. |
| pRS313 | CEN, HIS3, vector | (Sikorski and Hieter, 1989) |
| pRS316 | CEN, URA3, vector | (Sikorski and Hieter, 1989) |
| pRS416 | CEN, URA3, vector | (Sikorski and Hieter, 1989) |
| pBC111 | CEN, LEU2, vector | (Iida et al., 2007) |
| CHp282 | pRS416 PMET25-GFP | Chau Huynh, this laboratory |
| pLB215 | pRS416 PMET25-Ypk1 | (Niles et al., 2012) |
| pAX53 | pRS416 PMET25-Ypk1(K376A) | This study |
| pAX50 | BG1805 Ypk1(L424A) | This study |
| pFR203 | pGEX4T-1 Orm1(1-85) | (Roelants et al., 2011) |
| pBT6 | pGEX6P-1 Fps1(1-255) | This study |
| pBT7 | pGEX6P-1 Fps1(531-669) | This study |
| pBT12 | pGEX6P-1 Smp1 | This study |
| pAX55 | pGEX6P-1 Lcb3(1-79) | This study |
| pAX56 | pGEX6P-1 Cdc1(1-41) | This study |
| pAX58 | pGEX6P-1 Her1(1-224) | This study |
| pAX59 | pGEX6P-1 Rts3 | This study |
| pAX62 | pGEX6P-1 Fkh1 | This study |
| pAX63 | pGEX6P-1 Yhp1 | This study |
| pAX66 | pGEX6P-1 YNR014W | This study |
| pAX67 | pGEX6P-1 YHR097C | This study |
| pAX94 | pGEX6P-1 Mds3(545-1016) | This study |
| pAX131 | pGEX4T-1 Lac1(1-76) | This study |
| pAX132 | pGEX4T-1 Lac1(1-76)(S23A S24A) | This study |
| pFR291 | pGEX4T-1 Lag1(1-80) | This study |
| pAX133 | pGEX4T-1 Lag1(1-80)(S23A S24A) | This study |
| pAX134 | pGEX6P-1 Muk1(1-305) | This study |
| pAX215 | pGEX6P-1 Cyk3 | This study |
| pAX223 | pGEX6P-1 Gpt2(1-35) | This study |
| pAX224 | pGEX6P-1 Gpt2(570-743) | This study |
| pAX225 | pGEX6P-1 Bre5 | This study |
| pAX226 | pGEX6P-1 Npr1(1-437) | This study |
| pAX227 | pGEX6P-1 Pal1 | This study |
| pAX228 | pGEX6P-1 Ysp2(97-665) | This study |
| pAX229 | pGEX6P-1 Ysp2(1072-1282) | This study |
| pAX230 | pGEX6P-1 Atg21 | This study |
| pAX231 | pGEX6P-1 Pex31(250-462) | This study |
| pAX136 | pRS313 PLAC1-3xHA-Lac1 | This study |
| pFR273 | pRS316 PYPK1-Ypk1(D242A) | (Roelants et al., 2011) |
| pAX250 | pRS313 PTPI1-GFP-Atg8 | This study |
| pBCT-CCH1H | pBC111 PTDH3-Cch1 | (Iida et al., 2007) |