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. 2014 Nov 1;27(9):438–448. doi: 10.1089/vim.2014.0059

FIG. 1.

FIG. 1.

Design of the adeno-associated virus-like particle (AAVLP) display system and antigenicity testing of the AAVLPs. (A) OVA and control peptides were inserted between positions 587/588 of the AAV2 VP3 capsid protein. (B) Three-dimensional structure of VP3 capsid protein with the inserted peptide (as indicated by arrow). The image was created with The PyMOL Molecular Graphics System v1.3. (C) The AAVLP is composed of 60 copies of the VP3 capsid protein, also representing the inserted peptide (dark shapes) on the surface. The image was created with the PyMOL Molecular Graphic System v1.3. (D) AAVLPs were produced in HEK 293 cells by transfection of plasmid DNA, purified by FPLC, analyzed by SDS-PAGE for purity. Antigenicity was tested with enzyme-linked immunosorbent assay (ELISA). Therefore, AAVLP-OVA (E) and control AAVLP-TP18 (F) particles were coated on ELISA plates and incubated with polyclonal rabbit anti-OVA antibody or control rabbit antibody with irrelevant specificity, diluted as indicated at the x-axis. The y-axis shows the OD values. OD, optical density.