FIG. 3.
Subclass determination in single sera of immunized mice. ELISA plates were coated either with OVA (A), (C), and (E) to determine antigen-specific response or with wtAAVLP (B), (D), and (F) for the evaluation of anticarrier response. Mouse sera (PIS-3MIS) were diluted 1:100 for subsequent antibody subclass detection (IgG1, IgG2b, and IgG2a). PIS, pre-immune sera; 1MIS, first immune sera; 2MIS, second immune sera; 3MIS, third immune sera. x-Axis indicates the antigen used for vaccination; y-axis the mean OD405–490nm values.