Figure 1.
The GMN peptide modification confers unique in vitro transduction profiles compared to the parent vector, AAV2. (a) bEnd.3 mouse brain endothelial cells were transduced with AAV2, AAV-GMN, or control AAV2-PPS encoding eGFP reporter genes at an MOI of 105 vg/cell. 48 hours post-transduction, eGFP expression was measured by fluorescence microscopy. Scale bar is 250 μm (b) Prior to transduction, bEnd.3 cells were treated with the indicated enzymes to modify cell surface receptors. 48 hours after transduction, relative transduction was measured by fluorometric quantitation of eGFP expression. (c) CHO Pro5 and sialic acid deficient Lec2 cells were transduced with AAV-GMN, AAV2, or AAV5. 24 hours later, transduction was measured by eGFP fluorometry. In both graphs, transduction is presented as relative fluorescence units (RFU) of eGFP signal per μg of protein extract. Data shown are derived from ≥3 independent experiments. Error bars are mean ± SD. **P value <0.01; ns, not significant.