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. 2014 Oct 28;3(10):e205. doi: 10.1038/mtna.2014.56

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Copyright © 2014 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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The effect of Mcam silencing on migration of B16F10 cells. (a-c) The images of a wound healing assay were taken 48 hours after magnetofection (MF) and gene electrotransfer (EP) with pMCAM under visible light, 4× magnification and at 0, 8, and 15 hours, as designated, after the removal of a silicone cell-separator. Scale bar, 500 µm. (d) The kinetic analysis of cell migration. Bars represent AM ± SEM of the cell migration rate (N = 5). Asterisks indicate statistically significant differences between five control groups (untreated cells, only pSCR or pMCAM separately added to cells, MF or EP, MF + pSCR or EP + pSCR) and the group compared (MF + pMCAM or EP + pMCAM) (*P < 0.05). Crosses indicate statistically significant differences between MF + pMCAM and EP + pMCAM (^†P < 0.05). All data are normalized to the untreated cells.