Figure 1.

The nucleus contains the nucleoplasmic reticulum structure

The nuclear envelope was labeled with an antibody against lamin-A (green) in SkHep1 (A), an adenocarcinoma liver cell line, and freshly isolated primary hepatocytes treated (B) or not treated (C) with staurosporine (STA) for 4 h, respectively. DNA was stained with propidium iodide. Note the presence of the nucleoplasmic reticulum (NR) in a cell line as well as in a primary culture. In the two top panels, the images represent three focal planes, depicting the three dimensionality of the NR. In the cell lineage used here, the NR seems to be constitutively present (A). In the primary culture of hepatocytes, the NR, not present under control condition, can be induced by drugs, such as STA (compare B with C), corroborating the findings that the NR is a dynamic and inducible structure. Bar in A = 5 μm; bars in B and C = 10 μm.