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. Author manuscript; available in PMC: 2014 Nov 3.
Published in final edited form as: Aging Cell. 2012 Apr 9;11(4):559–568. doi: 10.1111/j.1474-9726.2012.00817.x

Figure 6.

Figure 6

The histidine analogue histidyl hydrazide (AG/01) reduces the ratio of Aβ42/Aβ40, γ-secretase activity and HNE-modified Nct in the brains of AD mice. Seven month-old male 3xTg-AD mice were treated with AG/01 (20 mg/kg) or vehicle (PBS) intraperitoneally every other day for one month. (A–C) Aβ40 and Aβ42 levels, and the ratio of Aβ42/Aβ40 in the hippocampal and neocortical tissues of 3xTg-AD mice treated with PBS or AG/01. Values are the mean and S.D. (6 mice/group). **p < 0.01 vs. vehicle-treated mice. (D) Hippocampal and neocortical tissues were homogenized and γ-secretase activity was measured using fluorogenic substrates. The values shown are the means and S.D. (6 mice/group). *p < 0.05, **p < 0.01 vs. vehicle-treated mice. (E and F) Hippocampal and neocortical lysates were immunoprecipitated with anti-nicastrin antibody, and HNE-nicastrin conjugates were detected using an antibody against HNE-protein adducts. Signal intensities of HNE-Nct and total amounts of immunoprecipitated nicastrin were quantified and HNE-Nct/total Nct ratios were calculated. Values (panel F) are the mean and S.D. *p < 0.05 vs. vehicle-treated mice.