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. 2014 Oct 22;3(10):e75. doi: 10.1038/emi.2014.75

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Copyright © 2014 Shanghai Shangyixun Cultural Communication Co., Ltd

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Growth kinetics and attachment of viruses in human respiratory tissues ex vivo. Replication of the MDk/W452(H5N8) virus was monitored in human nasal respiratory epithelial (A) and lung (B) tissue explants and corresponding culture supernatants (C and D, respectively) starting at 12 hpi and at 24-h intervals thereafter. Growth kinetics were compared to those of the control HPAI A(H5N1) (En/W149 and MDk/W401) and 2009 pandemic CA/07(H1N1) viruses. The titers shown are means±SD from three independently performed experiments. Immunohistochemical detection of viral NP antigen was performed using nasal and lung tissue blocks incubated with CA/07(H1N1) (E and F), the control strain, or MDk/W452(H5N8) (G and H), the test virus. Arrows indicate positive immunostaining. Scale bars=50 µm. *P<0.05, ***P<0.0001 relative to MDk/W452(H5N8).