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Fluorescence-stained live/dead MC3T3-E1 cells. Live cells in (a) and (c) are indicated by green fluorescence staining with calcein-AM. Dead cells in (b) and (d) are indicated by red staining with EthD-1. (a) and (b) are 1 day after seeding, and (c) and (d) are 7 days after seeding. Scale bars = 500 μm.
