Figure 2.
tRNAi and mRNA: Codon-Anticodon Interaction and Predicted POUT-to-PIN Transition
(A) Cross-section of the 40S subunit showing the tRNAi in the P site and the mRNA in its channel. eIF1A, eIF1, and eIF2α are also shown.
(B) A detailed view of the codon and anticodon and surrounding elements that stabilize this interaction.
(C) Superposition of the PIN and POUT (Hashem et al., 2013) ternary complexes. eIF2γ has been omitted for clarity. The tRNAi in this complex is about 7 Å deeper in the P site than the tRNAi in the pm43S complex. The eIF2α residues Arg55 and Arg57 are shown as sticks.
See also Figure S5.