A: A549 cells were treated with CsiRNA or MUC1siRNA and harvested 72 hr after transfection. Lysates were analyzed by immunoblotting with anti-MUC1 N-ter and anti-β-actin.
B: A549 cells were left untransfected (MOCK) or transfected with MUC1siRNA or CsiRNA, incubated for 72 hr, and then treated with 10 μM of CDDP for 48 hr. Cells were stained with FITC-conjugated annexin V and analyzed by flow cytometry.
C: The results for MOCK (open bars), MUC1siRNA transfected (solid bars), or CsiRNA transfected (hatched bars) cells are expressed as the percentage apoptosis (mean ± SD of three independent experiments).