FIG 4 .

Expression of roGFP2 in C. trachomatis L2. HeLa cells were infected with the expression vector-transformed chlamydiae, and roGFP2 expression was detected by Western blotting and fluorescence microscopy. The cell lysates prepared from untransformed, or transformed chlamydia-infected cells were isolated by SDS-PAGE and then transferred to nitrocellulose membrane. roGFP2 expression was detected with an anti-GFP antibody, and the MOMP was used as a loading control (A). The roGFP2 fluorescence from chlamydiae within inclusions was also observed by fluorescence microscopy at 16 hpi and 48 hpi (B and C). Scale bars are 23 µm.