FIG 6 .

Cross-linking of MOMP during the chlamydial developmental cycle. HeLa cells were infected with wild-type C. trachomatis L2 and lysed at the different times postinfection (see details in Materials and Methods). An equal amount of cell lysate containing chlamydial proteins was subjected to nonreducing (A) or reducing (B) SDS-PAGE, and the MOMP was detected by immunoblotting with an anti-MOMP antibody. The monomer form of MOMP under the nonreducing conditions was normalized with the reduced MOMP under the reducing condition (C). The monomer form of MOMP is indicated with asterisks (*). Where indicated, infected cells were treated with DTT (10 mM) or H₂O₂ (10 mM) for 10 min before being subjected to Western blotting.