FIG 6 .

UL7 induces STAT3 and ERK1/2 phosphorylation. Cell lysates from HAEC transduced with AdGFP or AdUL7 (A), infected with TR wtUL7HA or TR stopUL7HA for 96 h (B), and stimulated with pUL7Fc (C) were subjected to SDS-PAGE followed by immunoblotting. The membranes were decorated with antibodies specific for phospho-STAT3 and phospho-ERK1/2. Immunodetection was performed with total STAT3, ERK1, and actin serving as the internal controls. (D) Cells were treated with increasing concentrations of recombinant pUL7Fc in the presence or absence of UL7 and IL-6 neutralizing antibodies (Ab) for 24 h. ELISA was used to quantify the amount of IL-6 secreted from HAEC. Cell lysates were analyzed by Western blotting with specific antibodies for phospho-STAT3, total STAT3, and actin.