Figure 5.

Signaling pathways involved in the release of GLP-1 and PYY by OA. Cells were treated with OA (10 μM) (A,B) or 8-pCPT-2′-O-Me-cAMP (Epac L, 10 μM) (C,D) for 30 min in the presence or absence of inhibitors of PI hydrolysis (U73122, 10 μM), PKA (myristoylated PKI, 1 μM), or Ca²⁺ chelator (BAPTA-AM, 10 μM). Release of GLP-1 and PYY into the medium was measured by ELISA and the results are expressed as pg/100 ml. Treatment of cells with PKI, U73122 or BAPTA-AM alone had no significant effect on basal release. Values are mean ± s.e.m. of 6 experiments. ^**p < 0.001 vs. basal.