Figure 6.

Inhibition of OA-induced GLP-1 and PYY release by H₂S. Cells were incubated with OA (10 μM) in the presence or absence of various concentrations of L-cysteine, an activator of endogenous CSE and CBS, and H₂S donors NaHS or GYY4137. In some experiments, L-cysteine (100 mM) was co-incubated with the CSE inhibitor, DL-propargylglycine (PPG). GLP-1 (A) and PYY (B) release into the medium was measured by ELISA and the results are expressed as pg/100 ml. Values are mean ± s.e.m. of 4 experiments. ^**p < 0.01 vs. response to OA alone; ^#p < 0.05 vs. OA+L-cysteine (100 mM).