Figure 8.

Inhibition of OA-induced PI hydrolysis by H₂S. (A) Myo[³H]inositol labeled cells were incubated with OA (10 μM) in the presence or absence of L-cysteine (100 mM), NaHS (10 mM), or GYY4137 (10 mM). In some experiments L-cysteine was co-incubated with the CSE inhibitor, DL-propargylglycine (PPG). (B) Myo[³H]inositol labeled cells were incubated with 8-pCPT-2′-O-Me-cAMP (Epac L, 10 μM) in the presence or absence of L-cysteine (100 mM), NaHS (10 mM), or GYY4137 (10 mM). PI hydrolysis was measured by ion-exchange chromatography as increase in water soluble inositol formation. Results are expressed as cpm/mg protein. Values are mean ± s.e.m. of 3 experiments. ^**p < 0.001 vs. basal; ^#p < 0.05 vs. OA+L-cysteine.