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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2013 Nov 26;13(2):307–315. doi: 10.1158/1535-7163.MCT-13-0324

Figure 3. Elevated CIN in PDAC cells treated with the MPS1 inhibitor, NMS-P715.

Figure 3

FISH analysis with X chromosome (red) and chromosome 17 (green) centromeric probes in human BxPC3 or PANC-1 cells or a chromosome 11 probe (red) in mouse KRC cells (825-2 and 1170-4) after treatment with NMS-P715. Drug concentrations approximated preliminary IC50 values (data not shown). Cells were harvested after 48h (BxPC-3 and PANC-1) or 72h (825-2 and 1170-4). Percentage of cells exhibiting deviation from the modal chromosome number (%MD) provides an indirect measure of the CIN rate. Representative images of cells exhibiting either modal or non-modal signal numbers for each cell line are shown. Scale bar =10 μm. The modal chromosome numbers were: 2 for chromosomes X and 17 in BXPC-3 cells; 4 for chromosomes X and 17 in PANC-1 cells; 7 for chromosome 11 in 825-2 cells and 4 for chromosome 11 in 1170-4 cells. Significance was measured using a t-test.