Table 1.
Primer sequences used in RT-PCR and real-time PCR analysis.
| Gene | Direction | Sequences |
|---|---|---|
| Real-time PCR | ||
| IL-1β | F | 5-GTTGACGGACCCAAAAAGAT-3 |
| R | 5-CCTCATCCTGGAAGGTCCAC-3 | |
| iNOS | F | 5-CCCTTCCGAAGTTTCTGGCAGCAGC-3 |
| R | 5-GGCTGTCAGAGCCTCGTGGCTTTGG-3 | |
| COX-2 | F | 5-CACTACATCCTGACCCACTT-3 |
| R | 5-ATGCTCCTGCTTGAGTATGT-3 | |
| GAPDH | F | 5-CACTCACGGCAAATTCAACGGCAC-3 |
| R | 5-GACTCCACGACATACTCAGCAC-3 | |
|
| ||
| RT-PCR | ||
| IL-1β | F | 5-CAGGATGAGGACATGAGCACC-3 |
| R | 5-CTCTGCAGACTCAAACTCCAC-3 | |
| iNOS | F | 5-CCCTTCCGAAGTTTCTGGCAGCAGC-3 |
| R | 5-GGCTGTCAGAGCCTCGTGGCTTTGG-3 | |
| COX-2 | F | 5-CACTACATCCTGACCCACTT-3 |
| R | 5-ATGCTCCTGCTTGAGTATGT-3 | |
| GAPDH | F | 5-CAATGAATACGGCTACAGCA-3 |
| R | 5-AGGGAGATGCTCAGTGTTGG-3 | |