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. 2014 Oct 13;111(43):15390–15395. doi: 10.1073/pnas.1418334111

Fig. 4.

Fig. 4.

Reconstitution of a Stable CMG–Pol ε Complex. Glycerol gradient elution profiles of CMG alone (Bottom), Pol ε alone (Middle), and a mixture of Pol ε and CMG (Top). Fractions were collected from the bottom of the centrifuge tubes so that the largest molecular weight species were collected first, and a sample of each indicated fraction was separated by SDS/PAGE and stained with Coomassie Blue. The elution peak of each complex (as determined by densitometry shown in Fig. S6A) is indicated in the gels. Molecular mass size standards (thyroglobulin 670 kDa, γ-globulin 158 kDa, and ovalbumin 44 kDa) were run in a parallel gradient, and the migration peaks of each standard are indicated at the top of the gels. (Right) An expanded view of CMGE Fraction 14 showing the presence of all 15 CMGE subunits. The migration of each subunit is indicated to the right of the gels. Reconstitution of the CMG–Pol ε complex was demonstrated by glycerol gradient a total of six times.