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. 2014 Oct 13;111(43):15532–15537. doi: 10.1073/pnas.1411746111

Fig. 1.

Fig. 1.

Identification of a p53–XAF1 feedback loop. (A) A p53RE in the XAF1 promoter and reporter construction for luciferase assay. (B) p53 activation of the XAF1 promoter. Data represent the mean ± SD. **P < 0.01 (Student t test). (C) EMSA for p53 interaction with the p53RE. SD, serum deprivation (48 h); ET, etoposide (50 μM, 48 h). (D) Cytotoxic damage-specific XAF1 induction in HCT116 cells. IB, immunoblot. (E) Effect of XAF1 on HCT116 cell response to cytostatic damages. (F) Effect of XAF1 on p53 binding to its target promoters in HCT116 treated with etoposide (50 μM, 24 h). (G) XAF1 activation of p53 and its proapoptotic targets.