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. 2014 Oct 13;111(43):E4668–E4676. doi: 10.1073/pnas.1409642111

Fig. 3.

Fig. 3.

DSY3156 is a glycine betaine:cob(I)alamin methyltransferase. (A) UV-visible spectrum of 1.75 mM cobalamin reduced with Ti(III) citrate showing total change of absorbance vs. wavelength between 480 nm to 740 nm to demonstrate cob(I)alamin to methylcob(III)alamin (methylCbl) conversion in the presence of 5 mM GB and 50 μg of DSY3156. Spectra were gathered at 30-s intervals from 0 min to 9.5 min. Arrows indicate increasing or decreasing absorbance during course of reaction in different parts of spectrum. (B) Change in absorbance at 540 nm and 578 nm over time for an experiment in which the assay contained 50 mM GB, and at the arrow 50 μg of DSY3156 was added to the reaction mixture.