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. 2014 Oct 15;111(43):15450–15455. doi: 10.1073/pnas.1414856111

Fig. 4.

Fig. 4.

Plet-7::gfppest expression is elevated and detected early in lin-42 mutants. Plet-7::gfppest expression during midlarval development in wild-type and lin-42(lf) animals with exposure times indicated (ms). (A and B) Expression in the gut and pharynx of wild-type and lin-42(lf) animals, respectively. (C) GFP is precociously expressed in the seam of L2-stage lin-42(lf) mutants. (Inset) Magnification of two seam cells. GFP is not detected in the seam of wild type at this stage. (D–F) Fluorescence (Top) and DIC (Bottom) images of L2-molt stage animals. Plet-7::gfppest expression decreases during the L2 molt of wild type and lin-42(lf). Some GFP remains detectable in the pharynx and body wall muscle (BWM), particularly in the mutant. (G–I) Expression is detected in the seam of L3 stage wild-type animals and with greater intensity in lin-42(lf) mutants. (J and K) Fluorescence (Top) and DIC (Bottom) images of L3-molt stage animals. Plet-7::gfppest expression is not detected in the seam of L3m-stage animals, although BWM and pharynx expression remains visible in the mutant. (L) An early-L4 stage lin-42(lf) mutant lacks detectable GFP. (A–L) Scale bar, 50 μm.