Table 2.
Dissociation constants of full-length Tβ4 and truncates to actin and profilin:actin
| Tβ4 peptide | Tβ4:actin KdT (µM) | Profilin:actin:Tβ4 KdPT (µM) | Fold reduction KdPT/KdT |
| Full length | 0.32 ± 0.02 (0.27–0.37) | 7.3 ± 1.2 (4.5–10.1) | 23 |
| Pro4 truncate | 0.17 ± 0.02 (0.13–0.21) | 5.0 ± 0.8 (3.2–6.8) | 28 |
| Asp5 truncate | 2.4 ± 0.2 (2.0–2.9) | 13.0 ± 0.9 (10.9–15.1) | 5.4 |
| Met6 truncate | 2.3 ± 0.2 (1.7–2.9) | 5.8 ± 0.4 (4.9–6.7) | 2.5 |
Kd values are expressed as mean values ± SDs, and values in parentheses indicate 95% confidence intervals obtained by fitting the data in Fig. 2C using GraphPad Prism, version 4.00 (GraphPad Software; www.graphpad.com).