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. 2014 Oct 13;111(43):15526–15531. doi: 10.1073/pnas.1407717111

Fig. 4.

Fig. 4.

TGIF2 binds to the CDH promoter and recruits HDAC3 during EMT. (A) Schematic diagram showing the positions of two sets of primers designed to cover the promoter region of the CDH1 gene. (B) ChIP assays were performed with IgG and anti-TGIF2 antibody, followed by qPCR (mean ± SD, n = 3). ChIP samples were harvested from the nucleic lysate of SW480 cells treated as indicated for 72 h. (C) Western blot assays of immunoprecipitated samples of nucleic lysate with anti-TGIF2 antibody. Each sample was harvested after the cells were treated as indicated for 72 h. (D) ChIP assays were performed with IgG and anti-acetylated H3K9 antibody, followed by qPCR (mean ± SD, n = 3). ChIP samples were harvested from the nucleic lysate of SW480 cells treated as indicated for 72 h. Column values = average of at least three independent experiments; error bars represent SD from the mean of triplicate experiments. *P < 0.05.