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. Author manuscript; available in PMC: 2014 Nov 3.
Published in final edited form as: Contemp Clin Trials. 2013 May 3;35(2):55–67. doi: 10.1016/j.cct.2013.04.008
Aim 1. Determine the 95% confidence interval of the effect of the DASH diet on asthma control measured by change in the 7-item Juniper Asthma Control Questionnaire (ACQ) [45,46] at 6 months.
Aim 2. Estimate the confidence intervals of the effects of the DASH diet on specific manifestations of asthma that are indicative of current impairment (e.g., lung function, symptoms, and rescue medication use) and future risk (e.g, asthma exacerbations).
Aim 3. Explore changes in inflammatory markers important to asthma pathophysiology and their potential mediating effects on treatment response: total and differential leukocyte counts, fractional exhaled nitric oxide (FeNO), exhaled breath condensate pH, and serum cytokines (e.g., IL-5, IL-6, IL-13, IL-8, IL-17, TNF-α, and IFN-γ).
Aim 4. Conduct pre-specified subgroup analyses to examine genotypes ([e.g., polymorphisms on glutathione S transferase and microsomal epoxide hydrolase genes), phenotypes (e.g., age of onset, atopy, and obesity), and sociodemographic factors (sex, race/ethnicity, education, and income) that may modify the therapeutic effect of the DASH diet.