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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Mol Cell. 2014 Jan 30;53(4):606–616. doi: 10.1016/j.molcel.2014.01.003

Figure 3. Dicer rescue assay in Dicer knockout embryonic stem cells using ‘hDicer-specific helix’ mutants.

Figure 3

(A) Experimental scheme of the Dicer rescue assay.

(B) Sequences of ‘hDicer-specific helix’ mutants. Residues comprising the ‘hDicer-specific helix’ are colored in blue. Mutant residues are in red.

(C) Results of Taqman miRNA qPCR using miR-16 and miR-21 probes (left panel). The plasmids were transfected in two different concentrations (20 μg and 30μg). Western blot showed comparable expression of Dicer (right panel).