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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: J Comp Neurol. 2014 Apr 12;522(12):2691–2706. doi: 10.1002/cne.23576

Figure 1.

Figure 1

Sequential generation of neural progenitors, neurons, and astroglia in an established monolayer ES cell protocol is reproducible. A,B: Nestin expression decreases, as a proportion of total cells, from day in vitro (DIV) 14 to DIV 21. C,D: TuJ1 expression increases, as a proportion of total cells, from day 14 to day 21. E,F: GFAP expression begins by day 28. G: Quantification of nestin, TuJ1, and GFAP expression over the course of 28 days. Data are represented as mean ± s.e.m. (N = 3). Scale bar = 20 µm.