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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: J Comp Neurol. 2014 Apr 12;522(12):2691–2706. doi: 10.1002/cne.23576

Figure 4.

Figure 4

ES-derived, Ctip2-expressing neurons are immature, consistent with NeuN expression at midcorticogenesis in vivo. A: Approximately one-third of ES-derived, Ctip2-expressing neurons coexpress Map2 and NeuN in vitro (arrows, Ctip2/Map2/NeuN). B,C: In vivo, NeuN is normally expressed by one-third of E16.5 (B) and E18.5 (C) Ctip2-high neurons. D: By P6, in vivo, all retrogradely labeled SCPN coexpress Ctip2 and NeuN, indicating completion of a next stage of progressive maturation. A partial magenta-green version of this figure is available online in the Supporting Information. Scale bars = 10 µm in A; 150 µm in B (applies to B,C); 30 µm in D.