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. Author manuscript; available in PMC: 2014 Nov 3.
Published in final edited form as: Cell Host Microbe. 2008 Aug 14;4(2):123–133. doi: 10.1016/j.chom.2008.05.016

Figure 6. Knockdown of ERα in J-Lat 9.2 cells inhibits transcription from the PP5* promoter and activates transcription from the 5’LTR.

Figure 6

(A) Western blotting of endogenous ERα and GAPDH in cellular lysates from J-Lat 9.2, J-Lat 9.2 shER and a control J-Lat 8.4 shER cells as indicated. (B) RT-qPCR with a primer pair amplifying exon 2 of the PP5 gene was used to measure the inhibition of transcription from the PP5 promoter in J-Lat (white bars) and J-Lat shER (black bars) cells as indicated. (C-F) Histograms obtained with FACS of untreated (mock) and TNF-α-treated cells. Cell lines used are indicated on top of the panels. Numbers represent the percentage of GFP-positive cells indicated by horizontal lines.