Figure 6. H9 hEBs (formed under -ROCK/-spin condition) differentiated into pancreatic lineage at 18 days when treated with the pancreatic differentiation protocol.

(a) RT-PCR analysis demonstrated expression of key pancreatic lineage-specific genes. (b) Over 90% of the cells were insulin-positive, as evidenced by positive staining for dithizone (DTZ) in dark red. (c) Immunostaining of the cells revealed co-localization (in yellow) of insulin and C-peptide. Cell nuclei were stained in blue with DAPI.