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MIT group, heparin IN (hIN) group and low-molecular-weight heparin IN (lmwhIN) group were sensitized twice with Der p allergen subcutaneously on day 1 and day 8. Der p allergen was administered intratracheally on day 15. The hIN and lmwhIN groups were treated with heparin or lmw heparin intranasally from days 1 to 22. Splenocytes from sacrificed mice stimulated with Der p 16 ug/ml were cultured for 72 hours.
