Figure 6. Effect of normalization options on miR-203 expression in cervical tissues.

In a), it is shown that there is no significant effect on normalization between the use of the two most stable genes and the three genes. The differences in the expression levels of miR-203 were statistically significant with ANOVA when the use of the two and three most stable genes were compared with the use of each gene as a single normalizer. Graph b), shows the relative quantification of miR-203 in cervical tissues using a combination of miR-191 and miR-23a (from an analysis involving individual Ct values) as the normalizer. The error bars indicate a 95% confidence interval; **, p<0.01; ****, p<0.0001.