Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A. Western blot using anti-His6 monoclonal antibody in the presence (+) or absence (−) of the purified His6 tag-GmARI1 proteins. B. In vitro E3 ubiquitin ligase activity assay of GmARI1 protein. His6 tag-GmARI1 fusion proteins were assayed for E3 activity in the presence of rabbit E1, human E2 (UbcH5b), and ubiquitin (Ub). The left numbers denote the molecular masses of marker proteins in kilodaltons.
