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. 2014 Nov 3;9(11):e111258. doi: 10.1371/journal.pone.0111258

Figure 5. Transcript abundance of ethylene perception- and biosynthesis-related genes in “first berries” impaired in polar auxin transport.

Figure 5

(A) Effect of blocking polar auxin transport at 14 DAF using 50 µM N-1-naphthylphthalamic acid (NPA) on transcript abundance of VvETR1, VvETR2, VvERS2, VvCTR1, VvEIN3, VvERF12 genes assessed 2 days post treatment (DPT), at 16 DAF. (B) Effect of blocking polar auxin transport at 14 DAF using 50 µM NPA on transcript abundance of VvACO3, VvACS, VvACS1 and VvACS3 genes assessed 2 DPT, at 16 DAF. Transcript abundance is relative to the mean expression of the constitutive genes VvUBI1 and VvGPDH (for more details, see Material and Methods section). (C) Effect of blocking polar auxin transport at 14 DAF on abscission using 50 µM NPA assessed 2 DPT, at 16 DAF. Treatment was done immediately after berry number per cluster was registered, and remaining berries were registered for percentage (%) of berry abscission estimation (for berry number see Table 2). Only “first berries” were analyzed. Asterisk indicates that differences are significant between NPA-treated and control berries. Significance was assessed using p value <0.05 (n = 3). Error bars are STERR±.