Table 1. Primers used for quantitative real-time reverse transcriptase-PCR assays.
mRNA targeted | Sequence (5′→3′)(a) | Ref |
COX-1 | GTGCTGGGGCAGTGCTGGAG (F) | [15] |
TGGGGCCTGAGTAGCCCGTG (R) | ||
COX-2 | GCTGTACAAGCAGTGGCAAAG (F) | [16] |
GCGTTTGCGGTACTCATTGAGA (R) | ||
TNF-α | ATGAGCACAGAAAGCATGATC (F) | [17] |
TACAGGCTTGTCACTCGAATT (R) | ||
IL-17 | GCTCCAGAAGGCCCTCAGA (F) | [18] |
AGCTTTCCCTCCGCATTGA (R) | ||
IL-10 | CCAAGCCTTATCGGAAATGA (F) | [17] |
TTTTCACAGGGGAGAAATCG (R) | ||
IFN-γ | ACAATGAACGCTACACACTGCAT (F) | [19] |
TGGCAGTAACAGCCAGAAACA (R) | ||
FAS | ATGCACACTCTGCGATGAAG (F) | [20] |
CAGTGTTCACAGCCAGGAGA (R) | ||
FAS-L | ATTGAAGAAGACACCTTTACACTCATT (F) | [21] |
TGTCTCAAAAACGGCCTCTGT (R) | ||
18s rRNA | AACACGGGAAACCTCACCC(F) | [17] |
CCACCAACTAAGAACGGCCA (R) |
Primers were purchased from Invitrogen Inc. and were designed according to the DNA sequence information available for Mus musculus (M. musculus blast server BLAST Server Database at www.sanger.ac.uk).
F, forward primer; R, reverse primer.