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. 2014 Nov 3;9(11):e111408. doi: 10.1371/journal.pone.0111408

Figure 1. S. dysenteriae infected rat ileal loop model stimulates BMP/SMAD pathway.

Figure 1

A) Expression of BMP2 transcript level in S. dysenteriae infected rat ileal loop tissue at 4 hr and 8 hr and untreated (UT). BMP2 mRNA level was normalized with GAPDH. The values obtained with untreated group are referred to as 1. Data are the mean ± SD. (n = 3). *, p<0.05 B) S. dysenteriae infected rat ileal loop lysates were probed for indicated proteins. Tubulin was used as an internal control. C) Expression of CDX2 transcript level in S. dysenteriae infected rat ileal loop tissue at 4 hr and 8 hr. CDX2 mRNA level was normalized with GAPDH. The values obtained with untreated (UT) group are referred to as 1. Data are the mean ± SD. (n = 3). *, p<0.05 D) Nuclear pSMAD1/5/8 and cytoplasmic SMAD4 expression were increasing at 4 hr and 8 hr than in untreated (UT) in the villus region of the S. dysenteriae infected tissue sections. Nuclear expression of CDX2 was increased in the same conditions as described above. Scale bar represents 50 µM.