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. Author manuscript; available in PMC: 2014 Nov 3.

Published in final edited form as: Methods Mol Biol. 2012;889:51–71. doi: 10.1007/978-1-61779-867-2_5

Assessment of MO efficacy by in vitro transcription and translation. An expression construct encoding zebrafish Nrf2a (30) was transcribed and translated in vitro in the absence of any MO (lane 1) or in the presence of Nrf2-MO (500 nM; lane 2) or a control MO (500 nM; lane 3). Translated Nrf2 protein was labeled with [³⁵S]methionine.

Assessment of MO efficacy by in vitro transcription and translation. An expression construct encoding zebrafish Nrf2a (30) was transcribed and translated in vitro in the absence of any MO (lane 1) or in the presence of Nrf2-MO (500 nM; lane 2) or a control MO (500 nM; lane 3). Translated Nrf2 protein was labeled with [³⁵S]methionine.