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. Author manuscript; available in PMC: 2014 Nov 3.
Published in final edited form as: Methods Mol Biol. 2012;889:51–71. doi: 10.1007/978-1-61779-867-2_5

Figure 8.

Figure 8

Rescue of PCB-126-induced pericardial edema by Ahr-MO. Embryos were microinjected with 2 nl of 0.18 mM Ahr2-MO at the 1-4 cell stage, screened for incorporation of the fluorescently tagged MO at 24 hpf, and exposed in triplicate groups of 50 embryos to either 5 nM of PCB-126 or a DMSO solvent control from 24-48 hpf. At 72 hpf, embryos were mounted in 3% methylcellulose and imaged. Embryos exposed to DMSO show no deformities (left column). Non-injected and control-MO injected embryos exposed to PCB-126 suffer from pericardial edema, while Ahr2 morphant embryos are protected from this deformity (right column).