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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: Chembiochem. 2014 Aug 26;15(16):2353–2356. doi: 10.1002/cbic.201402343

Figure 2.

Figure 2

Capture/release of fluorescently labelled target oligonucleotides. (A) For each target sequence, a biotinylated capture oligonucleotide was designed. (B) The kinetics of release were measured using 8 nt capture/release oligonucleotides and a molar excess of release oligonucleotide ranging from 0-1000X. (C) Capture/release oligonucleotides containing different toehold lengths were used to measure the release kinetics with a molar excess of 1000X release oligonucleotide. Fluctuations in the 8 nt data are likely a result of noise in the assay due to handling of small volumes of magnetic particles. (D) Sequential release of two target oligonucleotides was performed from aliquots of the same sample using both combinations of release oligonucleotide order. (E) The fluorescence signal for both Alexa 488 and Texas Red was measured in all four release samples.