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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: Chembiochem. 2014 Aug 26;15(16):2353–2356. doi: 10.1002/cbic.201402343

Figure 4.

Figure 4

Selective release of a DNA oligomer hybridized to a microarray. (A) schematic of the DNA array after hybridization with a FAM-labelledoligonucleotide; (B) FAM and (C) Cy3 images of the surface; (D) schematic of the array after addition of a Cy3-labeled release oligonucleotide containing a toehold complementary to the fern sequence; (E) FAM and (F) Cy3 images taken after this exchange illustrating that the replacement of the FAM sequence with the Cy3 release oligonucleotide occurs only at features containing the designated toehold. The fern in panel E (green, negative image) shows the absence of FAM-labelled target oligonucleotide, while the fern in panel F (red, positive image) shows the presence of the displacing Cy3-labeled release oligonucleotide. The fern depicted in (E) and (F) is 9 mm in length and is comprised of individual DNA features that are 14 × 14 μm in size. We have observed no differences in elution efficiency from identical array features. Images (B), (C), (E) and (F) were enhanced for visibility (contrast+40% and brightness+40%).