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. Author manuscript; available in PMC: 2015 Jun 6.
Published in final edited form as: Neuroscience. 2014 Mar 13;269:199–214. doi: 10.1016/j.neuroscience.2014.02.015

Figure 6. Effects of CV4 5TG on A2 Noradrenergic Nerve Cell Fos, Dopamine-β-Hydroxylase (DβH), Steroid Hormone Receptor, and Substrate Fuel Transporter Protein Expression and AMPK Activity in OVX+EP Female Rats.

Figure 6

Individual TH-ir A2 neurons were laser-catapult microdissected from 10 μm-thick sections of the caudal dorsal vagal complex of OVX+EP rats 2 hr after intra-CV4 administration of 5TG (200 ug; n=8) or SAL (n=8). For each treatment group, separate lysates of pooled n=50 A2 cells (n=12-13/rat) were analyzed by Western blot for Fos (Row A), DβH (Row B), phosphoAMPK (Row C); AMPK (Row D), estrogen receptor-alpha (ERα; Row E), ER-beta (ERβ; Row F), progesterone receptor (Row G), monocarboxylate transporter-2 (MCT2; Row H), glucose transporter-4 (GLUT4; Row I), glucose transporter-3 (GLUT3; Panel Row J), or α-tubulin (Row K). For each protein of interest, immunoblot analyses were performed in triplicate.