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. 2014 Nov 3;9(11):e111649. doi: 10.1371/journal.pone.0111649

Figure 1. Design of two hypoallergenic mutants.

Figure 1

MEM49 was constructed by substitution of 49 different amino acid residues within the nine Met e 1 epitopes to the homologous fish tropomyosin sequence. MED171 was constructed by deletion of epitopes E1 to E9 of Met e1. (A) Location of the IgE-binding epitopes in tropomyosin. The IgE epitopes designated as E1–E9 are shown in boxes and the location of the 49 amino acid residues in Met e 1 that are converted in MEM49 are also shown as one letter amino acid code. (B) Schematic representation of Met e 1, MEM49 and MED171. Epitopes E1 to E9 in Met e 1 are represented as black boxes and the number of amino acids in each epitope is indicated. Amino acid residue changes in MEM49 are shown as *. MED171 is a truncated peptide with the epitopes E1–E9 deleted. (C) SDS-PAGE of Met e 1, MEM49 and MED171 after Coomassie Blue staining. Note the 35 kDa molecular weight of Met e 1 and MEM49 and the expected smaller size of MED171 compared to Met e 1.