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. 2014 Nov 3;9(11):e111649. doi: 10.1371/journal.pone.0111649

Figure 3. In vitro and in vivo IgE reactivity and allergenicity of the hypoallergens.

Figure 3

Reactivity of (A) MEM49 and (B) MED171 to IgE from shrimp allergy patients (n = 8) and reactivity of (C) MEM49 and (D) MED171 to IgE of Met e 1-sensitized mice (n = 8) in ELISA. (E) In vivo IgE reactivity and allergenicity of Met e 1, MEM49 and MED171 as determined by PCA assay. Note that the in vitro and in vivo IgE reactivity and allergenicity of MEM49 and MED171 are significantly lower than those of Met e 1, as shown by the significantly lower absorbance value at 450 nm and absence of Evan’s blue dye extravasation.