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. 2014 Nov 3;9(11):e111809. doi: 10.1371/journal.pone.0111809

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© 2014 Feng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The linear relationship between the number of spirochetes and Green/Red fluorescence ratio. (B) Emission spectra of suspensions of various proportions of live and isopropyl alcohol-killed B. burgdorferi were obtained, and the Green/Red fluorescence ratios were calculated for each proportion of live/dead cells. The line is a least-square fit of the relationship between percentage of live bacteria and Green/Red fluorescence ratio.