. 2014 Nov 3;9(11):e111809. doi: 10.1371/journal.pone.0111809

Table 1. Comparison of data¹ of different methods for assaying B. burgdorferi viability.

Percent of live cells	MTT²(OD₅₆₅)	XTT(OD₄₅₀)	FDA³(λem)	LIVE/DEAD(Ratio _Green/Red)	Sytox/Hoechst(Ratio _Green/Blue)	SYBR Green I/PI(Ratio _Green/Red)
0%	0.131	2.894	26069	10.81	0.947	3.49
20%	0.132	2.894	25938	10.13	0.943	4.15
50%	0.147	3.024	26134	11.35	0.931	5.48
80%	0.161	3.051	29136	10.75	0.927	7.17
100%	0.157	2.978	30158	11.63	0.924	8.03
BSK-H	0.152	2.892	26984	11.41	0.958	2.03

Each value is the mean of three replicates.

The formazan was dissolved in 100 µL of DMSO for 15 min.

Plates were incubated in dark at 33°C for 45 min before being read. λem refers to fluorescence emission at 518 nm with 494 nm excitation.

Table 1. Comparison of data1 of different methods for assaying B. burgdorferi viability.