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. 2014 Nov 3;9(11):e111968. doi: 10.1371/journal.pone.0111968

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© 2014 Kollenberg et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Viruses were localized after a 5 d acquisition access period and 2 d discharge using fluorescent in situ hybridization. Left column: virus localization using specific probes (red); right column: transmitted light images. WmCSV in B. tabaci 63 (A, B) and in B. tabaci 95 (C, D); TYLCV in B. tabaci 63 (E, F) and B. tabaci 95 (G, H); WmCSV in B. tabaci 63 after fluorescent in situ hybridization and nuclei staining with DAPI (I, J). CA, caeca; FC, filter chamber; DM, descending midgut; AM, ascending midgut; HG, hindgut. Arrowheads: virus accumulations.