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. 2014 Oct 30;7:2021–2033. doi: 10.2147/OTT.S67998

Figure 5.

Figure 5

ZINC69391 inhibited glioma cell migration and invasion modulating actin cytoskeleton reorganization.

Notes: (A) LN229 cells were seeded in uncoated transwell chambers with or without ZINC69391, incubated for 18 hours, and quantified. Bars, standard error of the mean. *P<0.05; ***P<0.001 determined by analysis of variance cont. Dunnett’s multiple comparison test. (B) LN229 cells were seeded in Matrigel-coated transwell chambers with or without ZINC69391, incubated for 48 hours, and quantified. Bars, standard error of the mean. *P<0.05; **P<0.01; ***P<0.001 determined by analysis of variance cont. Dunnett’s multiple comparison test. (C) Representative micrographs taken at 1,000× showing inhibition of epidermal growth factor (EGF)-induced actin reorganization by ZINC69391 in LN229 cells. Cells were grown on cover slips, serum-starved for 16 hours (untreated panel), and treated for 1 hour with ZINC69391. After 15 minutes stimulation with EGF (100 ng/mL) (EGF control), cells were fixed and actin filaments were visualized with AlexaFluor555-phalloidin.